The VEGF IRESes are differentially susceptible to translation inhibition by miR-16.

Abstract : Experiments with EMCV (Encephalomyocarditis virus) internal ribosome entry sites (IRESes) have shown that microRNAs (miRs) are unable to inhibit IRES driven translation. However, it is accepted that miRs can inhibit translation through multiple mechanisms, only some of which require interaction with the 5' cap structure. In this report, we first validate the targeting of miR-16 to a predicted binding site in the VEGF 3'UTR. We developed a series of experiments to ascertain whether or not miR-16 can inhibit translation of transcripts driven by either of the VEGF IRESes. Our results indicate that cellular IRESes can be classified as both sensitive and insensitive to miR control. While VEGF IRES-A activity was not altered by miR-16 targeting to the 3'UTR, IRES-B was susceptible to miR-16 inhibition. Taken together with previous results that show that IRES-B selectively translates the CUG initiated VEGF-121 isoform, we can conclude that the existence of two differentially susceptible IRESes in the VEGF 5'UTR leads to even more complex regulatory control of VEGF isoform production. This study demonstrates for the first time the inhibition of cellular IRES driven translation by a miR.
Type de document :
Article dans une revue
RNA, Cold Spring Harbor Laboratory Press, 2009, 15 (2), pp.249-54. 〈10.1261/rna.1301109〉
Liste complète des métadonnées

http://www.hal.inserm.fr/inserm-00410605
Contributeur : Marie Francoise Simon <>
Soumis le : vendredi 21 août 2009 - 15:38:46
Dernière modification le : vendredi 28 août 2009 - 15:52:20

Lien texte intégral

Identifiants

Collections

Citation

Zeïneb Karaa, Jason Iacovoni, Amandine Bastide, Eric Lacazette, Christian Touriol, et al.. The VEGF IRESes are differentially susceptible to translation inhibition by miR-16.. RNA, Cold Spring Harbor Laboratory Press, 2009, 15 (2), pp.249-54. 〈10.1261/rna.1301109〉. 〈inserm-00410605〉

Partager

Métriques

Consultations de la notice

58