Fibroblast growth factor 1 induced during myogenesis by a transcription-translation coupling mechanism.

Abstract : Fibroblast growth factor 1 (FGF1) is involved in muscle development and regeneration. The FGF1 gene contains four tissue-specific promoters allowing synthesis of four transcripts with distinct leader regions. Two of these transcripts contain internal ribosome entry sites (IRESs), which are RNA elements allowing mRNA translation to occur in conditions of blockade of the classical cap-dependent mechanism. Here, we investigated the function and the regulation of FGF1 during muscle differentiation and regeneration. Our data show that FGF1 protein expression is induced in differentiating myoblasts and regenerating mouse muscle, whereas siRNA knock-down demonstrated FGF1 requirement for myoblast differentiation. FGF1 induction occurred at both transcriptional and translational levels, involving specific activation of both promoter A and IRES A, whereas global cap-dependent translation was inhibited. Furthermore, we identified, in the FGF1 promoter A distal region, a cis-acting element able to activate the IRES A-driven translation. These data revealed a mechanism of molecular coupling of mRNA transcription and translation, involving a unique process of IRES activation by a promoter element. The crucial role of FGF1 in myoblast differentiation provides physiological relevance to this novel mechanism. This finding also provides a new insight into the molecular mechanisms linking different levels of gene expression regulation.
Type de document :
Article dans une revue
Nucleic Acids Research, Oxford University Press, 2009, 37 (16), pp.5267-78. 〈10.1093/nar/gkp550〉
Liste complète des métadonnées

Littérature citée [49 références]  Voir  Masquer  Télécharger

http://www.hal.inserm.fr/inserm-00410389
Contributeur : Marie Francoise Simon <>
Soumis le : vendredi 21 août 2009 - 17:05:16
Dernière modification le : jeudi 14 décembre 2017 - 14:12:02
Document(s) archivé(s) le : mardi 15 juin 2010 - 20:56:16

Fichier

gkp550v1.pdf
Fichiers éditeurs autorisés sur une archive ouverte

Identifiants

Collections

Citation

Caroline Conte, Nadera Ainaoui, Aurélie Delluc-Clavières, Marie Khoury, Rania Azar, et al.. Fibroblast growth factor 1 induced during myogenesis by a transcription-translation coupling mechanism.. Nucleic Acids Research, Oxford University Press, 2009, 37 (16), pp.5267-78. 〈10.1093/nar/gkp550〉. 〈inserm-00410389〉

Partager

Métriques

Consultations de la notice

265

Téléchargements de fichiers

335