Nutritional regulation and role of peroxisome proliferator-activated receptor delta in fatty acid catabolism in skeletal muscle.

Abstract : Peroxisome proliferator-activated receptors (PPARs) are nuclear receptors primarily involved in lipid homeostasis. PPARdelta displays strong expression in tissues with high lipid metabolism, such as adipose, intestine and muscle. Its role in skeletal muscle remains largely unknown. After a 24-h starvation period, PPARdelta mRNA levels are dramatically up-regulated in gastrocnemius muscle of mice and restored to control level upon refeeding. The rise of PPARdelta is accompanied by parallel up-regulations of fatty acid translocase/CD36 (FAT/CD36) and heart fatty acid binding protein (H-FABP), while refeeding promotes down-regulation of both genes. To directly access the role of PPARdelta in muscle cells, we forced its expression and that of a dominant-negative PPARdelta mutant in C2C12 myogenic cells. Differentiated C2C12 cells responds to 2-bromopalmitate or synthetic PPARdelta agonist by induction of genes involved in lipid metabolism and increment of fatty acid oxidation. Overexpression of PPARdelta enhanced these cellular responses, whereas expression of the dominant-negative mutant exerts opposite effects. These data strongly support a role for PPARdelta in the regulation of fatty acid oxidation in skeletal muscle and in adaptive response of this tissue to lipid catabolism.
Type de document :
Article dans une revue
BBA - Molecular Cell Research, Elsevier, 2003, 1633 (1), pp.43-50
Liste complète des métadonnées

http://www.hal.inserm.fr/inserm-00389948
Contributeur : Sarah Hamant <>
Soumis le : samedi 30 mai 2009 - 12:36:41
Dernière modification le : jeudi 11 janvier 2018 - 16:47:46

Identifiants

  • HAL Id : inserm-00389948, version 1
  • PUBMED : 12842194

Collections

Citation

Dorte Holst, Serge Luquet, Véronique Nogueira, Karsten Kristiansen, Xavier Leverve, et al.. Nutritional regulation and role of peroxisome proliferator-activated receptor delta in fatty acid catabolism in skeletal muscle.. BBA - Molecular Cell Research, Elsevier, 2003, 1633 (1), pp.43-50. 〈inserm-00389948〉

Partager

Métriques

Consultations de la notice

85