Endosome-to-cytosol transport of viral nucleocapsids.

Abstract : During viral infection, fusion of the viral envelope with endosomal membranes and nucleocapsid release were thought to be concomitant events. We show here that for the vesicular stomatitis virus they occur sequentially, at two successive steps of the endocytic pathway. Fusion already occurs in transport intermediates between early and late endosomes, presumably releasing the nucleocapsid within the lumen of intra-endosomal vesicles, where it remains hidden. Transport to late endosomes is then required for the nucleocapsid to be delivered to the cytoplasm. This last step, which initiates infection, depends on the late endosomal lipid lysobisphosphatidic acid (LBPA) and its putative effector Alix/AIP1, and is regulated by phosphatidylinositol-3-phosphate (PtdIns3P) signalling via the PtdIns3P-binding protein Snx16. We conclude that the nucleocapsid is exported into the cytoplasm after the back-fusion of internal vesicles with the limiting membrane of late endosomes, and that this process is controlled by the phospholipids LBPA and PtdIns3P and their effectors.
Type de document :
Article dans une revue
Nature Cell Biology, Nature Publishing Group, 2005, 7 (7), pp.653-64. 〈10.1038/ncb1269〉
Liste complète des métadonnées

Littérature citée [64 références]  Voir  Masquer  Télécharger

http://www.hal.inserm.fr/inserm-00381829
Contributeur : Sandrine Fraboulet <>
Soumis le : mardi 12 juillet 2011 - 17:32:47
Dernière modification le : lundi 5 février 2018 - 15:22:09
Document(s) archivé(s) le : jeudi 13 octobre 2011 - 02:20:08

Fichiers

inserm-00381829_edited.pdf
Fichiers produits par l'(les) auteur(s)

Identifiants

Collections

Citation

Isabelle Le Blanc, Pierre-Philippe Luyet, Véronique Pons, Charles Ferguson, Neil Emans, et al.. Endosome-to-cytosol transport of viral nucleocapsids.. Nature Cell Biology, Nature Publishing Group, 2005, 7 (7), pp.653-64. 〈10.1038/ncb1269〉. 〈inserm-00381829〉

Partager

Métriques

Consultations de la notice

327

Téléchargements de fichiers

901