A store-operated Ca2+ influx activated in response to the depletion of thapsigargin-sensitive Ca2+ stores is developmentally regulated in embryonic cortical neurons from mice.

Abstract : Store-operated channels (SOCs) are recruited in response to the release of Ca2+ from intracellular stores. They allow a voltage-independent entry of Ca2+ into the cytoplasm also termed capacitative Ca2+ entry (CCE). In neurons, the functional significance of this Ca2+ route remains elusive. Several reports indicate that SOCs could be developmentally regulated. We verified the presence of a CCE in freshly dissociated cortical cells from E13, E14, E16, E18 fetuses and from 1-day-old mice. Intracellular Ca2+ stores were depleted by means of the SERCA pump inhibitor thapsigargin. At E13, the release of Ca2+ from thapsigargin-sensitive compartments gave rise to an entry of Ca2+ in a minority of cells. This Ca2+ route, insensitive to voltage-gated Ca2+ channel antagonists like Cd2+ and Ni2+, was blocked by the SOC inhibitor SKF-96365. After E13 and on E13 cells kept in culture, there is a marked increase in the percentage of cells with functional SOCs. The lanthanide La3+ fully inhibited the CCE from neonatal mice whereas it weakly blocked the thapsigargin-dependent Ca2+ entry at E13. This suggests that the subunit composition of the cortical SOCs is developmentally regulated with La3+-insensitive channels being expressed in the embryonic cortex whereas La3+-sensitive SOCs are found at birth. Our data argue for the presence of SOCs in embryonic cortical neurons. Their expression and pharmacological properties are developmentally regulated.
Type de document :
Article dans une revue
Developmental Brain Research, Elsevier, 2005, 159 (1), pp.64-71. 〈10.1016/j.devbrainres.2005.07.001〉
Liste complète des métadonnées

http://www.hal.inserm.fr/inserm-00381727
Contributeur : Marco Canepari <>
Soumis le : mercredi 6 mai 2009 - 14:28:50
Dernière modification le : mercredi 6 mai 2009 - 16:55:35

Identifiants

Collections

CEA | UGA

Citation

Alexandre Bouron, Xavier Altafaj, Sylvie Boisseau, Michel De Waard. A store-operated Ca2+ influx activated in response to the depletion of thapsigargin-sensitive Ca2+ stores is developmentally regulated in embryonic cortical neurons from mice.. Developmental Brain Research, Elsevier, 2005, 159 (1), pp.64-71. 〈10.1016/j.devbrainres.2005.07.001〉. 〈inserm-00381727〉

Partager

Métriques

Consultations de la notice

93