Cerebral blood volume quantification in a C6 tumor model using gadolinium per (3,6-anhydro) alpha-cyclodextrin as a new magnetic resonance imaging preclinical contrast agent.

Abstract : In magnetic resonance imaging (MRI), cerebral blood volume (CBV) quantification is dependent on the MRI sequence and on the properties of the contrast agents (CAs). By using the rapid steady-state T(1) method, we show the potential of gadolinium per (3,6-anhydro) alpha-cyclodextrin (Gd-ACX), a new MRI paramagnetic CA (inclusion complex of Gd(3+) with per (3,6-anhydro)-alpha-cyclodextrin), for the CBV quantification in the presence of blood-brain barrier lesions. After biocompatibility and relaxivity experiments, in vivo experiments on rats were performed on a C6 tumor model with 0.05 mmol Gd-ACX/kg (<1/10 of the median lethal dose) injected at a 25 mmol/L concentration, inducing neither nephrotoxicity nor hemolysis. On T(1)-weighted images, a signal enhancement of 170% appeared in vessels after injection, but not in the tumor (during the 1 h of observation), in contrast to the 90% signal enhancement obtained with Gd-DOTA (a clinical MRI CA) injected at a T(1) isoefficient dose. This result shows the absence of Gd-ACX extravasation into the tumor tissue and its confinement to the vascular space. Fractional CBV values were found similar to Gd-ACX and Gd-DOTA in healthy brain tissue and in the contralateral hemisphere of tumor-bearing rats, whereas only Gd-ACX was appropriate for CBV quantification in tumor regions.
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Journal of Cerebral Blood Flow and Metabolism, Nature Publishing Group, 2008, 28 (5), pp.1017-29. 〈10.1038/sj.jcbfm.9600602〉
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http://www.hal.inserm.fr/inserm-00381358
Contributeur : Jean-Paul Issartel <>
Soumis le : mardi 5 mai 2009 - 15:25:13
Dernière modification le : vendredi 9 mars 2018 - 16:36:02

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Hana Lahrech, Adriana-Teodora Perles-Barbacaru, Soâd Aous, Jean-François Le Bas, Jean-Claude Debouzy, et al.. Cerebral blood volume quantification in a C6 tumor model using gadolinium per (3,6-anhydro) alpha-cyclodextrin as a new magnetic resonance imaging preclinical contrast agent.. Journal of Cerebral Blood Flow and Metabolism, Nature Publishing Group, 2008, 28 (5), pp.1017-29. 〈10.1038/sj.jcbfm.9600602〉. 〈inserm-00381358〉

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