PMID: identifiant
de la référence Pubmed : |
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 (17942559)  |
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| titre : |
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Serum-derived hepatitis C virus infection of primary human hepatocytes is tetraspanin CD81 dependent. |
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| auteur(s) : |
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Sonia Molina1, 2, Valerie Castet1, Lydiane Pichard-Garcia1, Czeslaw Wychowski3, Eliane Meurs4, Jean-Marc Pascussi1, Camille Sureau5, Jean-Michel Fabre1, 6, Antonio Sacunha7, Dominique Larrey1, 8, Jean Dubuisson3, Joliette Coste1, 2, Jane Mckeating9, Patrick Maurel ( ) 1, Chantal Fournier-Wirth1, 2 |
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| laboratoire : |
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| titre abrégé : |
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CD81 and HCV infection of primary hepatocyte |
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| résumé : |
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Hepatitis C virus-positive serum (HCVser, genotypes 1a to 3a) or HCV cell culture (JFH1/HCVcc) infection of primary normal human hepatocytes was assessed by measuring intracellular HCV RNA strands. Anti-CD81 antibodies and siRNA-CD81 silencing markedly inhibited (>90%) HCVser infection irrespective of HCV genotype, viral load, or liver donor, while hCD81-large intracellular loop (LEL) had no effect. However, JFH1/HCVcc infection of hepatocytes was modestly inhibited (40 to 60%) by both hCD81-LEL and anti-CD81 antibodies. In conclusion, CD81 is involved in HCVser infection of human hepatocytes, and comparative studies of HCVser versus JFH1/HCVcc infection of human hepatocytes and Huh-7.5 cells revealed that the cell-virion combination is determinant of the entry process. |
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| domaine : |
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Sciences du Vivant/Médecine humaine et pathologie/Hépatologie et Gastroentérologie
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langue du texte
intégral : |
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Anglais |
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| ISSN : |
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1098-5514 |
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| type de publication : |
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Articles dans des revues avec comité de lecture |
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| DOI : |
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10.1128/JVI.01443-07 |
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| journal : |
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| Journal of Virology (J Virol) |
| Publisher |
American Society for Microbiology |
| ISSN |
0022-538X |
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| Audience : |
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internationale |
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| date de publication : |
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01/2008 |
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date de publication
électronique : |
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17/10/2007 |
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| volume : |
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82 |
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| numéro : |
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1 |
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| page, identifiant, ... : |
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569-74 |
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| Descripteur(s) MeSH : |
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Adolescent – Adult – Aged – Antigens – CD – Cells – Cultured – Female – Gene Silencing – Hepacivirus – Hepatocytes – Humans – Male – Middle Aged – RNA – Viral – Receptors – Virus – Virus Internalization |
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