Efficient temporally-controlled targeted mutagenesis in smooth muscle cells of the adult mouse.

Abstract : To generate temporally-controlled targeted somatic mutations selectively and efficiently in smooth muscles, we have established a transgenic SMA-Cre-ER(T2) mouse line in which the expression of the Tamoxifen-dependent Cre-ER(T2) recombinase is under the control of a large genomic DNA segment of the mouse smooth muscle alpha actin (SMA) gene, contained in a Bacterial artificial chromosome (Bac). In this transgenic mouse line, Cre-ER(T2)-mediated recombination of LoxP-flanked target DNA is strictly Tamoxifen-dependent, and efficient in both vascular and visceral smooth muscle cells. Moreover, with the exception of few cardiomyocytes, LoxP-flanked DNA excision is restricted to smooth muscle cells. Thus, SMA-Cre-ER(T2) mice should be of great value to analyze gene function in smooth muscles, and to establish new animal models of human smooth muscle disorders.
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Genesis, Wiley-Blackwell, 2009, 47 (1), pp.14-8. 〈10.1002/dvg.20448〉
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http://www.hal.inserm.fr/inserm-00357169
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Soumis le : jeudi 29 janvier 2009 - 16:31:06
Dernière modification le : jeudi 11 janvier 2018 - 06:22:06

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Olivia Wendling, Jean-Marc Bornert, Pierre Chambon, Daniel Metzger. Efficient temporally-controlled targeted mutagenesis in smooth muscle cells of the adult mouse.. Genesis, Wiley-Blackwell, 2009, 47 (1), pp.14-8. 〈10.1002/dvg.20448〉. 〈inserm-00357169〉

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