PMID: identifier of
Pubmed reference: |
 |
 (18519648)  |
|
| title: |
|
Somatic diversification in the absence of antigen-driven responses is the hallmark of the IgM+ IgD+ CD27+ B cell repertoire in infants. |
|
| author(s): |
|
Sandra Weller1, Maria Mamani-Matsuda1, Capucine Picard2, 3, Corinne Cordier4, Damiana Lecoeuche1, Frédéric Gauthier5, Jean-Claude Weill ( ) 1, Claude-Agnès Reynaud () 1 |
|
| laboratory: |
|
|
|
| abstract: |
|
T cell-dependent immune responses develop soon after birth, whereas it takes 2 yr for humans to develop T cell-independent responses. We used this dissociation to analyze the repertoire diversification of IgM(+)IgD(+)CD27(+) B cells (also known as "IgM memory" B cells), comparing these cells with switched B cells in children <2 yr of age, with the aim of determining whether these two subsets are developmentally related. We show that the repertoire of IgM(+)IgD(+)CD27(+) B cells in the spleen and blood displays no sign of antigen-driven activation and expansion on H-CDR3 spectratyping, despite the many antigenic challenges provided by childhood vaccinations. This repertoire differed markedly from those of switched B cells and splenic germinal center B cells, even at the early stage of differentiation associated with mu heavy chain expression. These data provide evidence for the developmental diversification of IgM(+)IgD(+)CD27(+) B cells, at least in very young children, outside of T cell-dependent and -independent immune responses. |
|
| subject: |
|
Life Sciences/Immunology
|
|
| fulltext language: |
|
English |
|
| ISSN: |
|
0022-1007 |
|
|
| publication format: |
|
Article in peer-reviewed journal |
|
| DOI: |
|
10.1084/jem.20071555 |
|
| journal: |
|
Journal of Experimental Medicine / The Journal of Experimental Medicine |
|
| Audience: |
|
international |
|
| publication date: |
|
2008-06-09 |
|
| submission date: |
|
2008-06-02 |
|
| volume: |
|
205 |
|
| issue: |
|
6 |
|
| page, identifiant, ...: |
|
1331-42 |
|
|
| keyword(s) of author: |
|
"somatic hypermutation" – "splenic marginal zone" – "Ig repertoire" |
|
| MeSH Descriptor(s): |
|
Antigens – CD – Antigens – CD27 – B-Lymphocyte Subsets – B-Lymphocytes – Gene Rearrangement – Humans – Immunoglobulin D – Immunoglobulin M – Immunoglobulin Variable Region – Immunoglobulin mu-Chains – Immunologic Memory – Infant – Lymphocyte Activation – Spleen – T-Lymphocytes – Transcription – Genetic – Variation (Genetics) |
|
| contract, financing: |
|
Ligue Nationale contre le Cancer |
|
