PMID: identifier of
Pubmed reference: |
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 (15824086)  |
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| title: |
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Contribution of DNA polymerase eta to immunoglobulin gene hypermutation in the mouse. |
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| author(s): |
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Frédéric Delbos1, Annie De Smet1, Ahmad Faili1, Said Aoufouchi1, Jean-Claude Weill ( ) 1, Claude-Agnès Reynaud () 1 |
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| laboratory: |
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| 1: |
Developpement du Systeme Immunitaire |
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| INSERM : U783 – Université Paris V - Paris Descartes |
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| Fac de Medecine Necker-Enfants Malades PARIS V 156, Rue de Vaugirard 75730 PARIS CEDEX 15 |
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| France |
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| abstract: |
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The mutation pattern of immunoglobulin genes was studied in mice deficient for DNA polymerase eta, a translesional polymerase whose inactivation is responsible for the xeroderma pigmentosum variant (XP-V) syndrome in humans. Mutations show an 85% G/C biased pattern, similar to that reported for XP-V patients. Breeding these mice with animals harboring the stop codon mutation of the 129/Olain background in their DNA polymerase iota gene did not alter this pattern further. Although this G/C biased mutation profile resembles that of mice deficient in the MSH2 or MSH6 components of the mismatch repair complex, the residual A/T mutagenesis of pol eta-deficient mice differs markedly. This suggests that, in the absence of pol eta, the MSH2-MSH6 complex is able to recruit another DNA polymerase that is more accurate at copying A/T bases, possibly pol kappa, to assume its function in hypermutation. |
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| subject: |
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Life Sciences/Immunology
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| fulltext language: |
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English |
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| ISSN: |
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0022-1007 |
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| publication format: |
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Article in peer-reviewed journal |
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| DOI: |
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10.1084/jem.20050292 |
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| journal: |
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Journal of Experimental Medicine / The Journal of Experimental Medicine |
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| Audience: |
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international |
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| publication date: |
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2005-04-18 |
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| submission date: |
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2005-04-11 |
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| volume: |
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201 |
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| issue: |
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8 |
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| page, identifiant, ...: |
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1191-6 |
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| keyword(s) of author: |
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immunoglobulin genes – somatic hypermutation – polymerase eta |
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| MeSH Descriptor(s): |
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Animals – B-Lymphocytes – Base Pair Mismatch – Base Sequence – DNA Repair – DNA-Directed DNA Polymerase – Genes – Immunoglobulin – Mice – Knockout – Molecular Sequence Data – Peyer's Patches – Somatic Hypermutation – Animals |
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| contract, financing: |
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Ministère de la Recherche (A.C.I. Biologie du Développement et Physiologie Intégrative); Ligue Nationale Contre le Cancer; Fondation Princesse Grace de Monaco |
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