Two-dimensional gel electrophoresis (2DGE) images play a major role in techniques for protein separation. The registration of 2DGE images is considered as one of key elements in protein identification. This paper proposes a single adaptive registration scheme for 2DGE images. Within the scheme, three registration methods based on the combined use of global linear affine and local nonlinear thin-plate spline (TPS), Demons, B-spline algorithms are explored and compared. The results show that the method using multi-resolution B-spline is preferred.