Role of collagenase in mediating in vitro alveolar epithelial wound repair. - Inserm - Institut national de la santé et de la recherche médicale Accéder directement au contenu
Article Dans Une Revue Journal of Cell Science Année : 1999

Role of collagenase in mediating in vitro alveolar epithelial wound repair.

Résumé

Type II pneumocytes are essential for repair of the injured alveolar epithelium. The effect of two MMP collagenases, MMP-1 and MMP-13 on alveolar epithelial repair was studied in vitro. The A549 alveolar epithelial cell line and primary rat alveolar epithelial cell cultures were used. Cell adhesion and cell migration were measured with and without exogenous MMP-1. Wound healing of a cell monolayer of rat alveolar epithelial cell after a mechanical injury was evaluated by time lapse video analysis. Cell adhesion on type I collagen, as well as cytoskeleton stiffness, was decreased in the presence of exogenous collagenases. A similar decrease was observed when cell adhesion was tested on collagen that was first incubated with MMP-1 (versus control on intact collagen). Cell migration on type I collagen was promoted by collagenases. Wound healing of an alveolar epithelial cell monolayer was enhanced in the presence of exogenous collagenases. Our results suggest that collagenases could modulate the repair process by decreasing cell adhesion and cell stiffness, and by increasing cell migration on type I collagen. Collagen degradation could modify cell adhesion sites and collagen degradation peptides could induce alveolar type II pneumocyte migration. New insights regarding alveolar epithelial cell migration are particularly relevant to investigate early events during alveolar epithelial repair following lung injury.
Fichier principal
Vignette du fichier
243.pdf (251.41 Ko) Télécharger le fichier
Loading...

Dates et versions

inserm-00186823 , version 1 (19-11-2009)

Identifiants

  • HAL Id : inserm-00186823 , version 1
  • PUBMED : 9858477

Citer

Emmanuelle Planus, Stéphane Galiacy, Michael Matthay, Valérie M. Laurent, Jelena Gavrilovic, et al.. Role of collagenase in mediating in vitro alveolar epithelial wound repair.. Journal of Cell Science, 1999, 112 ( Pt 2), pp.243-52. ⟨inserm-00186823⟩
121 Consultations
189 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More