Fear of crowds is a symptom found in agoraphobia (among others) and which has both visual and auditory relevant components. In order to examine the potential interest of auditory-visual virtual reality to treat fear of crowds, we investigated the efficiency of an auditory-visual virtual environment in evoking affective reaction. We conducted an evaluation test with healthy participants sensitive to the fear of crowds to explore the influence of auditory-visual virtual environment on affective reactions. Our application involves both high fidelity visual stimulation and interactive 3D sound. Our specific presentation of animated crowds of humans creates an environment that is highly arousing, suggesting that manipulating the sensory presentation together with the spatial location of stimulation might provide a way to modulate affective reactions.